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. 2018 May 23;9(6):269. doi: 10.3390/genes9060269

Figure 2.

Figure 2

Distribution and copy number variation of repetitive DNAs in the A. latifasciata genome. (a) Fluorescent in situ hybridization mapping of four selected elements (DNA/hAT, Bel/Pao, Gypsy, and L2) on A. latifasciata metaphasic chromosomes, from a B+ specimen. Probes were PCR-labeled by biotin-dUTP, and the signal was detected by fluorescein isothiocyanate (FITC, green). Red arrowheads indicate the B chromosomes. The signal intensity is higher in the B chromosome than in the A complement. (b) qPCR of the Bel/Pao and Gypsy elements detected by the RepeatExplorer pipeline. Sample number 04 had no B chromosome (B-), while the others had at least one B (B+). The data show that B+ individuals had higher copy numbers than B- individuals, corroborating the previous results. GDR: Gene dosage ratio.