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. 2018 Jun 15;6(2):13. doi: 10.3390/jdb6020013

Figure 2.

Figure 2

Brief (3 h) FGF treatment promotes transcription of early developmental genes and maintains SHH expression in the former ZPA domain. (A) Top pathways and biological processes affected 3 h after FGF treatment. Purple bars indicate top pathways affected that differed from the 3 h transcriptome analysis. (B) Fold change of selected targets treated with FGF for 3 h compared to PBS treatment via RT-qPCR (p < 0.05). The y-axis shows the relative normalized gene expression. Assays were performed in triplicate and at least two independent experiments were performed. Expression of each gene was compared to a PBS control (p < 0.05 for each comparison). (C) WMISH validation of selected targets regulated by FGF after 3 h exposure. Top panel shows upregulation of respective genes, while the bottom panel consists of PBS-treated limbs. n = 6 embryos per gene for FGF treatment and 3 embryos per gene for PBS. White bars highlight the proximal extension of the SHH expression domain in the FGF-treated limb.