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. 2018 Jun 15;6(2):13. doi: 10.3390/jdb6020013

Figure 5.

Figure 5

The LHX2 expression pattern supports a role for maintaining distal posterior SHH expression during progressive limb outgrowth. (A) Left panel shows endogenous FGF8 (arrow) and SHH (arrowhead) expression in a HH23 limb bud. Middle panel shows endogenous LHX2 expression. Right panel is a composite of all three expression patterns. LHX2 expression is restricted to the distal mesoderm in the developing limb subjacent to the FGF8-expressing AER. Importantly, SHH is expressed only within the posterior boundary of the LHX2 expression domain. The overlapping expression pattern is consistent with a role for LHX2 in facilitating SHH expression in the developing limb. (B) After 3 and 24 h of FGF2 exposure, no appreciable ectopic LHX9 expression is observed (n = 3 embryos per treatment group for each time point). PBS beads were used as controls. Note that endogenous LHX9 expression is restricted to the anterior mesoderm.