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. 2001 Nov;12(11):3538–3549. doi: 10.1091/mbc.12.11.3538

Figure 2.

Figure 2

Septins and Int1p localize as spirals in INT1-filaments. Examples of images taken in the equatorial plane of a cell are noted by an asterisk (C and D). (A) YJB5001 grown in glucose and expressing Cdc12-GFP. (B) YJB3527 (cla4Δ) expressing Cdc12-GFP. (C) YJB5001 grown on galactose and thus expressing Cdc12-GFP and Int1p. Serial optical sections of one cell are shown. Bar (shown in C), 5 μm, is representative for A–F. (D) YJB5766 expressing Cdc3-GFP and Int1p. (E) YJB2681 expressing Sep7-GFP and Int1p. (F) YJB3362 expressing Int1-GFP for 6 or 12 h of growth on galactose, as indicated. (G) YJB5505 expressing both Cdc12-YFP and Int1-CFP after 12 h of INT1 induction. (H) Indirect immunofluorescence micrographs of YJB3363, after 12 h of INT1-HA induction, with the use of anti-HA and anti-Cdc3p antibodies as indicated. Bar (shown in H), 5 μm, is representative of G–H.