Figure 4.

Cdc12p septin is required for the establishment and maintenance of spirals. Bar, 10 μm. Fluorescence micrographs of cdc12–6 strains YJB4840 and YJB3542 expressing Int1-GFP (left) or Cdc3-GFP and Int1p (right). (A) Strains were pregrown at 37°C for 1 h to inactivate Cdc12–6p, subcultured to galactose-containing medium at 37°C to induce INT1 expression, and photographed after 12 h of growth. (B) Strains were pregrown in galactose at 23°C to induce INT1 and generate spirals, shifted to 37°C to inactivate Cdc12–6p, and photographed after 12 h at 37°C. Bar, 5 μm.