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Expression of INT1 alters the localization of other bud-neck–associated proteins. DIC and fluorescence micrographs of strains expressing INT1 and Myo1-GFP (YJB5170) (A), Hsl1-GFP (YJB5174) (B), or Gin4-GFP (YJB5172) (C). Cells not forming polarized buds in response to INT1 expression (black arrows) have normal localization of the GFP-fusion proteins at the mother-bud neck (white arrows). Bar, 10 μm.
