Table 3.

Effect of INT1 expression on the bud site selection pattern of S. cerevisiae strains

Strain	Ploidy	Plasmid	% Axial	% Bipolar	% Random
YJB1876	Haploid	pBM272	84	14	2
YJB1875	Haploid	pGAL1-INT1	40	8	52
YJB1844	Diploid	pBM272	3	84	13
YJB1843	Diploid	pGAL1-INT1	<1	5	94

The indicated strains were grown overnight to saturation in minimal medium without uracil, lacking glucose, but containing raffinose (2%). Growth in raffinose is sufficient to induce a small amount of INT1 expression from pGAL1-INT1. Cells were subcultured 1:10 into fresh medium containing galactose to fully induce INT1 expression, grown for 5 h, and stained with Calcofluor white to visualize bud scars. A total of 200 cells was scored for each strain.