Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2018 May 23;5(2):40. doi: 10.3390/bioengineering5020040

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2018 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

PMC Copyright notice

Fluorescent microscopic photos of anti-VE-Cadherin, Cy3 (red) immunofluorescence staining of HPAEC endothelial cells in the microfluidic device after finishing an experiment (nuclei stained with Hoechst) (blue). (a) Anti-VE-Cadherin Staining of the HPAEC endothelial cells within the microfluidic channel after an experiment. Red arrow shows edge of channel. VE-Cadherin can be detected on the surface of all endothelial cells and shows adherence junction expression; (c) Anti-VE-Cadherin staining of the HPAEC endothelial cells on top of the porous membrane of the microfluidic device after an experiment. VE-Cadherin can be detected on the surface of all of the endothelial cells and shows adherence junction expression. (b,d) Negative control for (a,c) show that method was conducted appropriately. Dilution of antibodies: 1st antibody anti VE-Cadherin 1:200, and 2nd antibody Cy3 anti rabbit 1:500). Scale bar: 50 µm.