Figure 8.

Rescue of the phenotype of mutant ltbsg1 by 24-EBL and the feedback regulation of BR-related genes. (A) Phenotypic observation of WT (left) and mutant ltbsg1 (right) at three-leaf stage treated with different doses of 24-EBL (0.000 to 1.000 μM). Bar = 1.5 cm; (B) The expression of LTBSG1 in leaf treated with 0.01 μM 24-EBL; (C) Quantification of endogenous brassinolide contents of WT and mutant ltbsg1; (D) The expression levels of genes related to BR biosynthesis in young panicles of WT and mutant ltbsg1; (E) The expression levels of genes related to BR signaling in young panicles of WT and mutant ltbsg1. The rice OsActin gene was used as an internal control. Values represent the means ± SD of three biological replicates (n = 3). ** p < 0.01.