Figure 1.

Representative transmission electron microscopy images of procyclic forms (PCF) (A) and bloodstream forms (BSF) (C) of Trypanosoma brucei showing contacts between acidocalcisomes and the mitochondria of the parasites. Acidocalcisomes appear as rounded organelles containing electron-dense material that adheres to one side of the membrane and are seen adjacent to the mitochondrion double membrane. The contact sites could be observed in both life cycle forms and are indicated by red arrows in the insets at higher magnification. Representative super resolution structured illumination images of T. brucei PCF (B) and BSF (D) trypanosomes subjected to proximity ligation assay. The red fluorescent signals indicated by the white arrows confirm the existence of membrane contact sites between acidocalcisomes and the mitochondria in these parasites. Only some of them are labeled with arrows. Scale bar in B also applies to D. Reproduced with permission from reference [92].