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Casein zymography of C. ulcerans culture supernatants. Samples were separated using non-reducing, casein-containing gels. After renaturation and incubation in reaction buffer, the gels were stained with Coomassie brilliant blue. Protease activity results in clear bands, which are indicated by arrows. Apparent molecular weights of marker proteins (Dual Colour, Biorad, Munich, Germany) are indicated.
