Figure 4.

Method for Generating 3D models from Tilt Stacks. (A) As tomograms can be generated from the tilted images; (B) individual structures can be segmented at different layers within the stack by regions of similar intensity; (C) A cross section of the reconstruction highlights the nanopore walls; (D) Reconstructed layers can merge the bottom orifice with a thinly floored pore due to similar intensities; (E) requiring manual adjustment to the narrowest orifice; (F) Segmented nanopores in a volume stack, viewed on the largest orifice; (G) Reconstructed nanopores (red) or the nanomembrane (blue inset, inverse of G) can then be exported into other software for visualization or simulation purposes.