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Hematoxylin and eosin (HE) staining of full-thickness skin equivalents (ftSEs). The ftSE morphology was evaluated at different time points. Images show representative cuts of ftSEs of (a,g) dermis only, (b,h) dermis with fibrin gel added on top, and (c,i) after seven days, (d,j) 10 days, (e,k) 17 days, and (f,l) 24 days of ALI culture. The skin equivalents developed a multilayered and stratified epithelium during this 24-day period. (a–f) Scale bar: 500 µm. (g–l) Scale bar: 50 µm.
