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. 2018 Jun 7;5(2):43. doi: 10.3390/bioengineering5020043

Table 1.

List of antibodies used for immunofluorescence staining.

Antibodies Dilution in PBS Manufacturer
Collagen I, mouse anti-human, 5.9 mg/mL 1:100 Sigma 1
Cytokeratin 10, mouse anti-human, 1 mg/mL 1:100 Chemicon 2
Cytokeratin 15, rabbit anti-human, 100 µg/mL 1:100 Chemicon 2
Filaggrin, mouse anti-human, 0.2 mg/mL 1:50 Thermo Fisher 3
IgG–CF 488, goat anti-rabbit, 2 mg/mL 1:200 Biotium 4
IgG–CF 594, goat anti-mouse, 2 mg/mL 1:200 Biotium 4
Vimentin, IgG rabbit anti-human, 200 µg/mL 1:100 Santa Cruz 5

1 Sigma-Aldrich, St. Louis, MO, USA. 2 Merck, Darmstadt, Germany. 3 Thermo Fisher, Schwerte, Germany. 4 Biotium, Fremont, CA, USA. 5 Santa Cruz Biotechnology, Dallas, TX, USA.