Figure 5.

A chromosomal translocation alters the positioning of centromeric α-satellite DNA. Scheme of the t(11;14)(q13;q32) translocation present in JVM-2 cells with resulting loss of dark G bands (a). Metaphase chromosomes (stained red) were hybridized with a painting probe for chromosome 11 (stained green). The normal chromosome 11 and the 2 translocation products, t(11;14) and t(14; 11), are indicated (b). The α-satellite signals corresponding to the normal (11) and translocated (t(11;14)) chromosomes were scored for association with the lamina (f_LamTot), and the nucleolus (f_NucTot) SD is indicated. Predicted distribution frequencies were calculated with the use of the model depicted in Figure 4.