Table 3A.
Immunohistochemical expression of differentiation markers in the uterine epithelium following neonatal exposure to GEN or DES
| Age (mo)
|
|||||||
|---|---|---|---|---|---|---|---|
| 2–3
|
18
|
||||||
| Marker | Site | CON | GEN | DES | CON | GEN | DES |
| SIX1 | Cervix: non-neoplastic | 13/13 (100%) |
10/10 (100%) |
9/9 (100%) |
9/9 (100%) |
10/10 (100%) |
12/12 (100%) |
| Labeling score | 2.2 | 1.9 | 2.0 | 1.9 | 1.4 | 1.6 | |
|
|
|
|
|||||
| Endometrium: non-neoplastic | 1/13 (8%) |
9/10c (90%) |
9/9c (100%) |
0/9 (0%) |
10/10c (100%) |
12/12c (100%) |
|
| Labeling score | 0.1 | 1.1 | 1.2 | 0.0 | 2.2 | 2.6 | |
|
|
|
|
|||||
| Endometrium: neoplastic | na | na | na | na | 10/10 (100%) |
12/12 (100%) |
|
| Labeling score | na | na | na | na | 2.0 | 2.5 | |
|
| |||||||
| P63 | Cervix: non-neoplastic | 13/13 (100%) |
10/10 (100%) |
9/9 (100%) |
9/9 (100%) |
10/10 (100%) |
12/12 (100%) |
| Labeling score | 2.7 | 3.0 | 2.7 | 2.6 | 2.7 | 2.8 | |
|
|
|
|
|||||
| Endometrium: non-neoplastic | 1/13 (8%) |
8/10c (80%) |
8/9c (89%) |
4/9 (44%) |
10/10a (100%) |
12/12b (100%) |
|
| Labeling score | 0.1 | 0.8 | 0.9 | 0.4 | 2.0 | 2.5 | |
|
|
|
|
|||||
| Endometrium: neoplastic | na | na | na | na | 10/10 (100%) |
12/12 (100%) |
|
| Labeling score | na | na | na | na | 2.1 | 1.8 | |
Abbreviations: CON, control; GEN, genistein; DES, diethylstilbestrol; na, not applicable. Cervix includes stratified squamous epithelium, mucification layer if present, and transition zone with uterine body; endometrium includes glands and epithelium lining central lumen. IHC labeling score represents a qualitative average (0-4) within each group.
Letters indicate
a
P<0.05,
b
P<0.01, and
c
P<0.001 compared with corresponding age- matched control group using a two-tailed Fisher exact test.