Table 3B.
Immunohistochemical expression of differentiation markers in the uterine epithelium following neonatal exposure to DES or GEN
| Age (mo)
|
|||||||
|---|---|---|---|---|---|---|---|
| 2–3
|
18
|
||||||
| Marker | Site | CON | GEN | DES | CON | GEN | DES |
| CK14 | Cervix: non-neoplastic | 13/13 (100%) |
10/10 (100%) |
9/9 (100%) |
9/9 (100%) |
10/10 (100%) |
12/12 (100%) |
| Labeling score | 4.0 | 4.0 | 4.0 | 4.0 | 4.0 | 4.0 | |
|
|
|
|
|||||
| Endometrium: non-neoplastic | 1/13 (8%) |
10/10c (100%) |
9/9c (100%) |
4/9 (44%) |
10/10a (100%) |
12/12b (100%) |
|
| Labeling score | 0.1 | 1.2 | 1.0 | 0.4 | 2.5 | 2.7 | |
|
|
|
|
|||||
| Endometrium: neoplastic | na | na | na | na | 10/10 (100%) |
12/12 (100%) |
|
| Labeling score | na | na | na | na | 3.5 | 3.2 | |
|
| |||||||
| CK18 | Cervix: non-neoplastic# | 9/13 (69%) |
0/10b (0%) |
2/9 (22%) |
1/9 (11%) |
5/10 (50%) |
5/12 (42%) |
| Labeling score | 0.9 | 0.0 | 0.2 | 0.1 | 0.5 | 0.4 | |
|
|
|
|
|||||
| Endometrium: non-neoplastic | 13/13 (100%) |
10/10 (100%) |
9/9 (100%) |
9/9 (100%) |
10/10 (100%) |
12/12 (100%) |
|
| Labeling score | 3.8 | 4.0 | 4.0 | 4.0 | 3.9 | 3.9 | |
|
|
|
|
|||||
| Endometrium: neoplastic | na | na | na | na | 10/10 (100%) |
12/12 (100%) |
|
| Labeling score | na | na | na | na | 3.1 | 2.8 | |
|
| |||||||
| Dual CK14/18 | Cervix: non-neoplastic#* | 6/10 (60%) |
0/10a (0%) |
1/9 (11%) |
4/9 (44%) |
0/10a (0%) |
2/11 (18%) |
| Labeling score | 0.8 | 0.0 | 0.1 | 0.4 | 0.0 | 0.2 | |
|
|
|
|
|||||
| Endometrium: non-neoplastic* | 4/9 (44%) |
10/10a (100%) |
9/9a (100%) |
2/9 (22%) |
10/10c (100%) |
10/11b (91%) |
|
| Labeling score | 0.4 | 1.2 | 1.1 | 0.2 | 1.0 | 0.9 | |
|
|
|
|
|||||
| Endometrium: neoplastic* | na | na | na | na | 9/9 (100%) |
11/11 (100%) |
|
| Labeling score | na | na | na | na | 2.1 | 2.1 | |
Abbreviations: CON, control; GEN, genistein; DES, diethylstilbestrol; na, not applicable. Cervix includes stratified squamous epithelium, mucification layer if present, and transition zone with uterine body; endometrium includes glands and epithelium lining central lumen. IHC labeling score represents average (0-4) within each group.
Tissues from some uteri were not present in section.
CK18 labeling in the cervix was most commonly observed in a superficial mucification layer, which was often not present in GEN or DES mice.
Letters indicate
P<0.05,
P<0.01, and
P<0.001 compared with corresponding age- matched control group using a two-tailed Fisher exact test.