Table 3C.
Immunohistochemical expression of differentiation markers in the uterine epithelium following neonatal exposure to DES or GEN
| Age (mo)
|
|||||||
|---|---|---|---|---|---|---|---|
| 2–3
|
18
|
||||||
| Marker | Site | CON | GEN | DES | CON | GEN | DES |
| CD44 | Cervix: non-neoplastic | 13/13 (100%) |
10/10 (100%) |
9/9 (100%) |
9/9 (100%) |
10/10 (100%) |
12/12 (100%) |
| Labeling score | 2.3 | 3.0 | 3.0 | 2.8 | 2.7 | 2.8 | |
|
|
|
|
|||||
| Endometrium: non-neoplastic | 6/13 (46%) |
10/10b (100%) |
9/9a (100%) |
9/9 (100%) |
10/10 (100%) |
11/12 (92%) |
|
| Labeling score | 0.6 | 1.1 | 1.4 | 1.2 | 1.0 | 1.0 | |
|
|
|
|
|||||
| Endometrium: neoplastic | na | na | na | na | 9/10 (90%) |
8/12 (67%) |
|
| Labeling score | na | na | na | na | 1.3 | 1.0 | |
|
| |||||||
| TUNEL | Cervix: non-neoplastic* | 7/10 (70%) |
10/10 (100%) |
9/9 (100%) |
8/8 (100%) |
9/9 (100%) |
10/11 (91%) |
| Labeling score | 0.9 | 1.2 | 1.0 | 1.4 | 1.0 | 1.0 | |
|
|
|
|
|||||
| Endometrium: non-neoplastic* | 7/9 (78%) |
10/10 (100%) |
9/9 (100%) |
9/9 (100%) |
9/10 (90%) |
11/11 (100%) |
|
| Labeling score | 1.7 | 1.2 | 1.2 | 1.8 | 0.9 | 1.0 | |
|
|
|
|
|||||
| Endometrium: neoplastic* | na | na | na | na | 9/10 (90%) |
10/11 (91%) |
|
| Labeling score | na | na | na | na | 0.9 | 0.9 | |
Abbreviations: CON, control; GEN, genistein; DES, diethylstilbestrol; na, not applicable. Cervix includes stratified squamous epithelium, mucification layer if present, and transition zone with uterine body; endometrium includes glands and epithelium lining central lumen. IHC labeling score represents average (0-4) within each group.
*
Tissues from some uteri were not present in section.
Letters indicate
a
P<0.05,
b
P<0.01, and
c
P<0.001 compared with corresponding age- matched control group using a two-tailed Fisher exact test.