Table 3D.
Immunohistochemical expression of differentiation markers in the uterine epithelium following neonatal exposure to DES or GEN
| Age (mo)
|
|||||||
|---|---|---|---|---|---|---|---|
| 2–3
|
18
|
||||||
| Marker | Site | CON | GEN | DES | CON | GEN | DES |
| ERα | Cervix: non-neoplastic | 13/13 (100%) |
10/10 (100%) |
9/9 (100%) |
9/9 (100%) |
10/10 (100%) |
12/12 (100%) |
| Labeling score | 2.4 | 2.0 | 2.0 | 2.8 | 2.0 | 2.3 | |
|
|
|
|
|||||
| Endometrium: non-neoplastic* | 12/12 (100%) |
10/10 (100%) |
9/9 (100%) |
9/9 (100%) |
10/10 (100%) |
12/12 (100%) |
|
| Labeling score | 3.2 | 3.9 | 3.9 | 3.3 | 3.7 | 4.0 | |
|
|
|
|
|||||
| Endometrium: neoplastic | na | na | na | na | 10/10 (100%) |
12/12 (100%) |
|
| Labeling score | na | na | na | na | 3.3 | 3.5 | |
|
| |||||||
| PGR | Cervix: non-neoplastic | 13/13 (100%) |
10/10 (100%) |
9/9 (100%) |
9/9 (100%) |
10/10 (100%) |
12/12 (100%) |
| Labeling score | 2.5 | 3.5 | 3.4 | 2.1 | 2.7 | 2.4 | |
|
|
|
|
|||||
| Endometrium: non-neoplastic* | 12/12 (100%) |
10/10 (100%) |
9/9 (100%) |
9/9 (100%) |
10/10 (100%) |
12/12 (100%) |
|
| Labeling score | 1.3 | 1.6 | 1.8 | 2.0 | 2.2 | 2.1 | |
|
|
|
|
|||||
| Endometrium: neoplastic | na | na | na | na | 10/10 (100%) |
12/12 (100%) |
|
| Labeling score | na | na | na | na | 1.8 | 1.7 | |
Abbreviations: CON, control; GEN, genistein; DES, diethylstilbestrol; na, not applicable. Cervix includes stratified squamous epithelium, mucification layer if present, and transition zone with uterine body; endometrium includes glands and epithelium lining central lumen. IHC labeling score represents average (0-4) within each group.
*
Tissues from some uteri were not present in section.
Letters indicate
a
P<0.05,
b
P<0.01, and
c
P<0.001 compared with corresponding age-matched control group using a two-tailed Fisher exact test.