Table S1.

Primers for PD-1, CTLA-4, FOXP3, TGF-β, IL-10, IL-2Rα, CD28, and β-actin

Molecules	Primers
PD1-F	5′-GGTGTGAGGCCATCCACAA-3′
PD1-R	5′-CCATTCTGTCGGAGCCTCTG-3′
PD1-probe	5′-AGCCGATTAGCCATGGACAGTTG-3′
CTLA4-F	5′-GCTGACAGCCAGGTGACTGAA-3′
CTLA4-R	5′-TGAGCTCCACCTTGCAGATG-3′
CTLA4-probe	5′-CTGTGCGGCAACCTACATGATGG-3′
FOXP3-F	5′-AAGAGCCAGAGGACTTCCTCAA-3′
FOXP3-R	5′-CATGGCACTCAGCTTCTCCTT-3′
FOXP3-probe	5′-CAGGCGGACCATCTTCTGGATGA-3′
TGF-β-F	5′-CAACACATCAGAGCTCCGAGAA-3′
TGF-β-R	5′-GCTGAGGTATCGCCAGGAAT-3′
TGF-β-probe	5′-CTGCGTCTGCTGAGGCTCAAGTTA-3′
IL-10-F	5′-TGCCAAGCCTTGTCTGAGATG-3′
IL-10-R	5′-CCACGGCCTTGCTCTTGTT-3′
IL-10-probe	5′-CAGACATCAAGGCGCATGTGAAC-3′
IL-2Rα-F	5′-ATGGCTGCAACCATGGAGAC-3′
IL-2Rα-R	5′-TCTGTTCCCGGCTTCTTACC-3′
IL-2Rα-probe	5′-TGATCAGCGTCCTCCTCCTGAGTG-3′
CD28-F	5′-CGCAGTGGCTCATGCTTGTA-3′
CD28-R	5′-TCTCATGCCTCAGCCTCTTG-3′
CD28-probe	5′-AGATCAGGACCAGCCTGGTCAAGA-3′
β-Actin-F	5′-ACGTTGCTATCCAGGCTGTG-3′
β-Actin-R	5′-CGCTCGGTGAGGATCTTCAT-3′
β-Actin-probe	5′-CGTACCACTGGCATCGTGATGGA-3′

Abbreviations: PD-1, programmed cell death 1; CTLA-4, cytotoxic T lymphocyte antigen 4; FOXP3, forkhead box P3; TGF-β, transforming growth factor-beta; IL-10, interleukin-10; IL-2Rα, interleukin-2 receptor alpha; CD28, cluster of differentiation 28.