Figure 3.

a. Extracts from intracellular (I) and extracellular (E) fractions of pulse-chased gga1Δ/gga2Δ cells transformed with either empty vector, wild-type Gga1p (wt), the Δ-ear deletion mutant, or the γ-ear chimera were immunoprecipitated with anti-CPY. The γ-ear chimera gives full rescue of CPY sorting, whereas the Δ-ear deletion mutant gives partial rescue. (b) Secretion of active α-factor was investigated using the halo assay. Again, the γ-ear chimera gives full rescue of the wild-type phenotype, and the Δ-ear deletion mutant gives partial rescue