Figure 7.

Localization of Gga1p at the electron microscope level. (a) vps4 cells expressing HA-tagged Gga1p on a 2μ plasmid were labeled with anti-HA followed by protein A coupled to 10 nm gold. Most of the label is associated with a multilamellar compartment with the characteristic appearance of the class E compartment. A coated vesicular profile is also labeled (arrowheads). (b) vps4 cells coexpressing HA-tagged Gga1p and myc-tagged Vps10p were double labeled for HA (10 nm gold, arrowheads) and myc (15 nm gold, arrows). The colocalization of the two proteins on the same multilamellar compartment confirms that in a vps4 mutant GGAs are targeted to the class E compartment. Scale bars, 200 nm.