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. Author manuscript; available in PMC: 2018 Jul 2.
Published in final edited form as: Am J Med Genet A. 2015 Jul 2;167A(11):2533–2540. doi: 10.1002/ajmg.a.37214

TABLE I.

Rescue of orc6 Deletion Mutant With Different orc6 Transgenes Under Native or tubulin Promoter

GFP-orc6-wild-type GFP-orc6-Y225F GFP-orc6-Y225S GFP-orc6-D224A/Y225A GFP-orc6-W228A/K229A GFP-orc6-200
Native + + − − − −
 promoter 92% (989) 50% (214) 0% (515) 0% (617) 0% (713) 0% (820)
tubulin + + + + − −
 promoter 91% (704) 55% (183) 16% (1281) 20% (838) 0% (489) 0% (504)

In all rescue experiments of the orc6 deletion mutant we used transgenes expressing fused GFP-Orc6 proteins. Six different constructs were designed. GFP was attached to the N-terminus of either wild-type Orc6 or one of the mutants. All constructs contained two active promoters-native orc6 promoter and GAL4 inducible UAS promoter. (+), the expression of transgene restores viability of orc6-deleted flies; (−), no rescue. Percentage of rescued flies was calculated based on expected segregation of phenotypes. Total progeny analyzed is shown in parentheses.