Table 2.
List of primary antibodies for histology
Antigen | Clone | Species | Source | Order # | Dilution | Incubation time |
---|---|---|---|---|---|---|
Total human tau | HT7 | mouse | Pierce Biotechnology, USA | MN1000 | 1:500 | overnight at 4 °C |
pThr231 | AT180 | mouse | Pierce Biotechnology, USA | MN1040 | 1:200 | overnight at 4 °C |
pSer202 + pThr205 | AT8 | mouse | Thermo Scientific, USA | MN1020 | 1:200 | overnight at 4 °C |
pSer199 | polyclonal | rabbit | Invitrogen Corporation, USA | 44-734G | 1:500 | 1 h at RT |
pTyr18 | 9G3 | mouse | MédiMabs, Canada | MM-0194-P | 1:1000 | 1 h at RT |
pSer396 | PHF13 | mouse | Cell Signaling Technology, USA | 9632 | 1:500 | 1 h at RT |
pSer262 | polyclonal | rabbit | MBL International, USA | AT-5020 | 1:250 | overnight at 4 °C |
pSer422 | 5.6.11 | mouse | Roche Diagnostic, Switzerland | N/A | 1:800 | overnight at 4 °C |