Table 3.
List of secondary antibodies
| Antibody | Conjugation | Source | Order # | To visualize |
|---|---|---|---|---|
| Donkey Anti-Mouse IgG H&L | AlexaFluor 555 | abcam, UK | ab150110 | pSer422 tau |
| pSer396 tau | ||||
| pSer202/ pThr205 tau | ||||
| Donkey Anti-Rabbit IgG H&L | DyLight 650 | abcam, UK | ab96922 | pSer199 tau |
| pSer262 tau | ||||
| Donkey Anti-Mouse IgG H&L | DyLight 550 | abcam, UK | ab98795 | pThr231 tau |
| Donkey Anti-Mouse IgG (H + L) | Cyanine Cy3 | Jackson ImmunoResearch, USA | 715–165-151 | Total human tau |
| Donkey Anti-Mouse IgG H&L | DyLight 650 | abcam, UK | ab98797 | pTyr18 tau |
| Dilution of all secondary antibodies was 1:500 | ||||