Table 1.

Yeast strains

Straina	Genotype	Source
L5683	MATα ura3-52	G. Fink
L5977	MATa ura3-52 his3 trp1	G. Fink
SKY723	MATa/MATα ura3-52/ura3-52 his3/his3 trp1/trp1
SKY750	MATa/MATα cdc28Δ∷TRP1/cdc28Δ∷TRP1ura3-52/ura3-52 his3/his3 trp1/trp1 [pCT3 CDC28]
SKY754	MATa ura3-52	Ahn et al., 1999
SKY757	MATa/MATα ura3-52/ura3-52	Ahn et al., 1999
SKY772	MATa ura3-52 leu2 clb2Δ∷LEU2	Ahn et al., 1999
SKY778	MATa/MATα ura3-52/ura3-52 leu2/leu2 clb2Δ∷LEU2/clb2Δ∷LEU2	Ahn et al., 1999
SKY786	SKY862 clb2Δ∷LEU2/clb2Δ∷LEU2
SKY862	SKY750 leu2/leu2
SKY940	SKY862 mih1Δ∷LEU2/mih1Δ∷LEU2
SKY942	SKY862 swe1Δ∷LEU2/swe1Δ∷LEU2
SKY962	SKY862 leu2∷GAL1-CLB2∷LEU2/leu2	Stueland et al., 1993
SKY979	MATα ura3-52 his3
SKY980	MATa ura3-52 trp1
SKY1057	MATa/MATα ura3-52/ura3-52 mih1Δ∷LEU2/mih1Δ∷LEU2
SKY1058	MATa/MATα ura3-52/ura3-52 swe1Δ∷LEU2/swe1Δ∷LEU2
SKY2030	SKY862 cln1Δ∷LEU2/cln1Δ∷LEU2
SKY2407	MATα ura3-52 cdc28-1N	Ahn et al., 1999
SKY2415	MATa ura3-52 cdc28-1N	Ahn et al., 1999
SKY2646	SKY750 GAL-CLN1∷kanMX6/GAL-CLN1∷kanMX6
SKY2665	MATa/MATα GAL-CLN1∷kanMX6b/GAL-CLN1∷kanMX6
SKY2666	MATa/MATα GAL-CLN2∷kanMX6/GAL-CLN2∷kanMX6
SKY2667	MATa/MATα swe1Δ∷LEU2/swe1Δ∷LEU2 GAL-CLN1∷kanMX6/CLN1
SKY2668	MATa/MATα swe1Δ∷LEU2/swe1Δ∷LEU2 GAL-CLN2∷kanMX6/CLN2
SKY2669	MATa/MATα SWE1-MYCc/SWE1-MYC
SKY2670	MATa/MATα SWE1-MYC/SWE1-MYC [pEG202 CDC28]
SKY2671	MATa/MATα SWE1-MYC/SWE1-MYC [pEG202 cdc28-E12G]
SKY2672	MATa/MATα SWE1-MYC/SWE1-MYC [pEG202 cdc28-E12K]
SKY2673	MATa/MATα swe1Δ∷LEU2/swe1Δ∷LEU2

^a All strains are Σ1287b derivatives and were generated in this study unless otherwise indicated. The his3, trp1, and leu2 alleles are hisG disruptions, as described by Liu et al. (1993). 

^b Constructed by PCR mutagenesis using pFA6a-kanMX6 plasmid (Wach et al., 1994). 

^c Constructed by PCR mutagenesis using pFA61-13Myc-kanMX6 plasmid (Longtine et al., 1998).