Table 3.
Phenotypes of cdc28 mutants with altered cyclin dosage
| Mutant | Polar morphogenesis when expressed in:
|
||||||||
|---|---|---|---|---|---|---|---|---|---|
| Wild type |
GAL-CLB2
|
clb2Δ
|
cln3Δ
|
GAL-CLN1
|
cln1Δ
|
swe1Δ
|
|||
| YPD | YPG | clb2Δ | cln3Δ | YPD | YPG | cln1Δ | swe1Δ | ||
| Wild type | +/− | + | + | ++++ | +/− | + | +++ | +/− | ++ |
| Glu12Gly | ++++ | ++ | + | L | ++ | +++ | +++++ | +/− | + |
| Val21Asp | +++ | ++ | + | L | + | ++ | +++ | +++ | ++++ |
| Ala55Thr | ++++ | ++ | + | L | ++ | ++ | +++ | ++++ | ++++ |
| Ile59Asn | ++++ | ++ | + | L | +++ | ++ | +++ | +++ | +++ |
| Glu89Lys | ++++ | ++ | + | L | ++ | ++ | +++ | +++ | ++++ |
| Phe116Leu | ++++ | ++ | + | L | ++++ | + | +++ | +++ | ++++ |
| Leu137Ile | +++ | ++ | + | L | ++ | + | +++ | +++ | ++++ |
| Phe202Tyr | ++++ | + | + | L | +++ | ++ | +++ | +++ | ++++ |
| Ile221Thr | +++ | ++ | + | L | ++ | ++ | +++ | +++ | +++ |
Shuffle diploid strains of the indicated genotypes were transformed with plasmid-borne cdc28ECP alleles. Polarized growth on YPD or YPG agar was assayed as described in MATERIALS AND METHODS. L, synthetic lethal.