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. 2018 May 22;8(7):2173–2179. doi: 10.1534/g3.118.200417

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Copyright © 2018 Ba et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Quality evaluation of ddRAD-Seq library construction. (A) Selection of enzymes for the construction of ddRAD libraries by evaluating cutting efficiency of restrictions enzymes 6 base recognition sites (i.e., PstI, NsiI, EcoRI and SacI) and 4 base recognition sites (i.e., MseI and MluCI). (B) Frequency distribution of restriction fragment length, including the expected 3,049,956 fragment sequences sheared from the reference genome, the observed 2,121,131 loci from ddRAD libraries and those containing SNPs.