Table 5. Deficiencies with published dorsal closure genes.
| Screen Name | Known Dorsal Closure Gene(s) | References | Known gene fully phenocopies Df? | Comments |
|---|---|---|---|---|
| Df(2R)02 | scaf | (Rousset et al. 2010) (Sorrosal et al. 2010) | Yes | Similar to published phenotype |
| Df(2R)03 | Src42A | (Tateno et al. 2000) | Unknown | Phenotype described from cuticle defects in combination with other mutants |
| Df(2R)04 | Src42A | (Tateno et al. 2000) | No | Phenotype described from cuticle defects in combination with other mutants, Df(2R)04 is more severe than Df(2R)03 |
| Df(2R)10 | ptc | (Jankovics et al. 2011) | No | Published phenotype is less severe |
| Df(2R)11 | Gγ1, ptc | (Yi et al. 2008) (Jankovics et al. 2011) | Unknown | Additive effect of 2 genes has not been tested |
| Df(2R)12 | Pkn | (Lu and Settleman 1999) | Unknown | Phenotype described from germline clones and cuticle defects |
| Df(2R)17 | Jra | (Riesgo-Escovar and Hafen 1997) | No | Published phenotype is less severe |
| Df(2R)18 | Jra | (Riesgo-Escovar and Hafen 1997) | Yes | Similar to published phenotype |
| Df(2R)20 | shn, acal | (Rusten et al. 2002) (Fernandez et al. 2007) (Ríos-Barrera et al. 2015) | Unknown | Additive effect of 2 genes has not been tested |
| Df(2R)21 | shn | (Rusten et al. 2002) (Fernandez et al. 2007) | No | Published phenotype is less severe |
| Df(2R)28 | Mos | (Sopko et al. 2014) | No | Phenotype described from cuticle defects with RNAi, Df(2R)28 is more severe than Df(2R)29 |
| Df(2R)29 | Mos | (Sopko et al. 2014) | Unknown | Phenotype described from cuticle defects with RNAi |
| Df(2R)32 | Ack-like | (Zahedi et al. 2008) | No | Published phenotype is less severe |
| Df(2R)33 | GstE14 | (Enya et al. 2014) | Unknown | Phenotype described from cuticle defects |
| Df(2R)35 | shot | (Takács et al. 2017) | No | Published phenotype is less severe |
| Df(2R)36 | shot | (Takács et al. 2017) | N/A | Problem with Df (see below) |
| Df(2R)37 | shot | (Takács et al. 2017) | No | Published phenotype is less severe |
| Df(2R)44 | scb, Arf51F | (Wada et al. 2007) (Jankovics et al. 2011) | Unknown | Additive effect of 2 genes has not been tested |
| Df(2R)45 | Zasp52 | (Ducuing and Vincent 2016) | No | Zasp52 published phenotype is more severe due to a suspected 2nd site lesion |
| Df(2R)46 | Zasp52 | (Ducuing and Vincent 2016) | No | Zasp52 published phenotype is more severe due to a suspected 2nd site lesion |
| Df(2R)47 | Rho1 | (Lu and Settleman 1999) (Rodriguez-Diaz et al. 2008) | Yes | Similar to published phenotype |
| Df(2R)48 | shark | (Fernandez et al. 2000) | No | Phenotype described from cuticle defects in germline clones, zygotic nulls survive to larval stages, Df does not hatch |
| Df(2R)50 | Cdk4 | (Sopko et al. 2014) | Unknown | Phenotype described from RNAi |
| Df(2R)52 | RhoGEF2 | (Azevedo et al. 2011) | Unknown | Phenotype described from germline clones |
| Df(2R)53 | RhoGEF2 | (Azevedo et al. 2011) | Unknown | Phenotype described from germline clones |
| Df(2R)57 | POSH | (Lennox and Stronach 2010) (Zhang et al. 2010) | Unknown | Published phenotype described from cuticle defects, has low penetrance (10–12%) |
| Df(2R)62 | ena | (Grevengoed et al. 2001) (Gates et al. 2007) | No | Published phenotype is less severe |
| Df(2R)63 | rib, cora, ena | (Nüsslein-Volhard et al. 1984) (Blake et al. 1998) (Lamb et al. 1998) (Grevengoed et al. 2001) (Gates et al. 2007) | Unknown | Additive effect of 3 genes has not been tested |
| Df(2R)69 | mir-311, mir-312 | (Leaman et al. 2005) | Unknown | Phenotype described from RNAi |
| Df(2R)70 | mir-311, mir-312 | (Leaman et al. 2005) | Unknown | Phenotype described from RNAi |
| Df(2R)71 | mir-311, mir-312 | (Leaman et al. 2005) | No | Df(2R)71 has more severe phenotype than Df(2R)69 and 70 |
| Df(2R)72 | shg | (Gorfinkiel and Martinez-Arias 2007) | No | Ecad-GFP rescues null completely (see Fig 12 and 13) |
| Df(2R)75 | Egfr | (Shen et al. 2013) | Yes | Similar to published phenotype |
| Df(2R)85 | gbb | (Chen et al. 1998) | Unknown | Phenotype described in combination with other mutants |
| Df(2R)90 | zip | (Jacinto et al. 2002) (Franke et al. 2005) | Yes | Similar to published phenotype |
| Df(2R)91 | Kr | (Jankovics et al. 2011) | No | Published phenotype is less severe |
| Df(2R)92 | Kr | (Jankovics et al. 2011) | Yes | Similar to published phenotype |
Deficiency phenotypes were compared with the phenotype(s) of the previously identified dorsal closure gene(s) deleted by the Df. Column 1 refers to the assigned Df number for the screen; the corresponding Bloomington stock number can be found in Appendix A. Column 2 lists the previously identified dorsal closure gene(s) removed by the Df. The third column lists the references in which these dorsal closure genes were identified. The fourth column lists whether the phenotype of the dorsal closure gene fully phenocopies the homozygous Df. The fifth column summarizes how the phenotypes compare to published phenotypes.