Table 2. The 18 compounds that showed promise in the screen and were considered pre-hits. These had a Z-score of >2 in 2 of 3 replicates in the primary screen.
| Common Name | Replicate 1 | Replicate 2 | Replicate 3 | Retest, Phase 1* | Retest, Phase 2 *** | Retest, Phase 3 *** | Retest, Phase 4 **** |
|---|---|---|---|---|---|---|---|
| CRUSTECDYSONE | 3.36 | 2.24 | 3.03 | Small study indicated that 100μM rescued developmental delay to pupation | Trend supported that small size was partially rescued | Trend supported that small size was partially rescued | Positive – conclusive evidence that larval developmental delay and pupal lethality was recued. |
| TETRACYCLINE HUDROCHLORIDE | 2.84 | 1.01 | 2.39 | Z > 2, 3X @50μM | Trend supported that small size was partially rescued | Negative | Negative |
| HARMINE | 3.37 | 1.92 | 2.41 | Z > 2 in >1 replicate | Trend supported that small size was partially rescued | Negative | Negative |
| SULFLURAMID | 4.45 | 6.22 | −0.01 | Z > 2, 3X @ 12.5μM | Larvae appeared larger than controls, but the measured areas were not. | Negative | Not tested |
| URSOCHOLANIC ACID | 8.61 | 7.51 | 1.85 | 25 and 50μM could not be tested b/c of insolubility | Larvae appeared larger than controls, but the measured areas were not. | Negative | Not tested |
| 5a-ANDROSTANE | 6.46 | −1.10 | 2.44 | Z > 2 in >1 replicate | Negative | Not tested | Not tested |
| IRIGENIN TRIMETHYLETHER | 0.73 | 5.62 | 4.90 | Z > 2 in >1 replicate | Negative | Not tested | Not tested |
| PATULIN | −0.43 | 2.22 | 2.63 | Z > 2 in >1 replicate | Negative | Not tested | Not tested |
| ANTIMONY POTASSIUM TARTRATE TRIHYDRATE | 2.30 | 0.57 | 2.66 | Z > 2 in >1 replicate | Negative | Not tested | Not tested |
| ALOGLIPTIN BENZOATE | 2.23 | 8.26 | −2.26 | Z > 2 in >1 replicate | Negative | Not tested | Not tested |
| CEFEPIME HYDROCHLORIDE | 3.25 | −0.12 | 3.85 | Z > 2, 2X, @50μM | Negative | Not tested | Not tested |
| PALMIDROL (5mM) | 3.95 | 1.01 | 5.46 | 10μM did not rescue developmental delay or pupal lethality at 21°C. | Not tested | Not tested | Not tested |
| THIOGUANINE, TIOGUANINE** | 3.51 | −0.91 | 0.80 | Z not >2 in > 1 replicate | Not tested | Not tested | Not tested |
| THIOGUANINE, TIOGUANINE | 0.91 | −0.02 | 4.97 | Z not >2 in > 1 replicate | Not tested | Not tested | Not tested |
| CAPTAN | 4.11 | −3.41 | 3.36 | Z not >2 in > 1 replicate | Not tested | Not tested | Not tested |
| AGARIC ACID | 3.16 | 1.90 | 4.05 | Z not >2 in > 1 replicate | Not tested | Not tested | Not tested |
| ZONISAMIDE | 0.42 | 3.19 | 2.55 | Z not >2 in > 1 replicate | Not tested | Not tested | Not tested |
| BROXALDINE | 2.05 | −1.05 | 6.62 | Z not >2 in > 1 replicate | Not tested | Not tested | Not tested |
| PICEID | 2.66 | 3.04 | 2.06 | Not tested | Not tested | Not tested | Not tested |
*
Phase 1 was 3 or 6 reps of a dose response (3.125, 6.25, 12.5, 25, and 50μM) in the primary screen/96 well format and/or tests for rescue of developmental delay (CRUSTECDYSONE and PALMIDROL).
**
The drug library had thioguanine in 2 different wells. *** Phase 2 and 3 tested whether promising pre-hits could rescue larval size at 10μM or 50μM in petri-dish experiments. ****Phase 4 tested whether promising pre-hits could rescue larval developmental delay and pupal lethality at 22.6° C.