Figure 4.
Colocalization of various chimeric GFPs with the ganglioside GM1. Colocalization of GM1 as detected with FITC-CTX (green) with acylated chimeric GFPs YesGFP, Src16GFP, and GAP-43GFP, as detected with the use of rabbit anti-GFP antibody followed by TR-conjugated donkey anti-rabbit antibody. Arrowheads indicate areas of plasma membrane colocalization, and arrows indicate endosomal/Golgi colocalization. Bars, 10 μm.
