Figure 5.

Colocalization of chimeric GFPs and endogenous p62^c-yes PTK with caveolin in COS-7 cells. COS-7 cells were transfected with plasmids encoding various acylated GFP chimeras and processed for indirect immunofluorescence analysis 24 h after transfection. The GFP chimeras were detected by incubation with mouse anti-GFP antibody followed by FITC-conjugated anti-mouse antibody. Endogenous p62^c-yes was detected with mouse anti-Yes antibody and FITC-conjugated donkey anti-mouse secondary antibody. The same cells were incubated with rabbit anti-caveolin antibody followed by TR-conjugated anti-rabbit secondary antibody. Arrowheads point to caveolin-GAP-43GFP Golgi colocalization, and arrows point to clusters of acylated GFP-containing structures and caveolin-containing structures. Bars, 10 μm.