Figure 7.

Solubilization of acylated chimeric GFPs expressed in COS-7 cells by 0.1 or 1% TX-100 and 60 mM OG at 4°C. COS-7 cells expressing various acylated and nonacylated GFP chimeras, and GFP alone, were solubilized in detergent at 4°C for 20 min and then separated into soluble (S) and resistant (P) fractions. Aliquots of soluble and resistant fractions were analyzed by immunoblotting with the use of rabbit anti-GFP and anti-caveolin antibodies. (A) Immunoblots show the distribution of the expressed proteins and endogenous caveolin in soluble and resistant fractions in the presence of indicated detergents. Plus signs indicate the type and concentration of detergent used to solubilize the cells. (B) Coomassie-stained PVDFs, corresponding to the immunoblots above, depict distribution of total cellular protein after detergent solubilization.