Column screening results using SFC/QqQMS with six different normal-phase columns including BEH, 2-EP, 2-PIC, DEA, DIOL, and 1-AA. A: Separation behavior of each lipid class. The data show the mean value of five replicated experiments. B: Ratio of W0.1 of each column to that of BEH column in each lipid class. Data were log2-transformed, and the mean value of five replicated experimental data was presented. W0.1, peak width of 10% peak height (second).