Fig. 1.

Schematic diagram outlining the in vivo assay to determine FAO in conscious mice. Radioactive lipid tracer (10⁷ DPM of ³H-palmitate and 10⁷ DPM of ¹⁴C-2-bromopalmitate) is administered through a jugular vein catheter at a flow rate of 20 µl/min for 5 min. Blood samples are collected throughout the infusion period and up to t = 12 min, after which the mouse is euthanized via pentobarbital injection. Tissues are collected and frozen in liquid nitrogen. Lipid extraction performed on blood and tissue samples allows for the separation of infused radioactive lipids from the β-oxidation by-product ³H₂O that accumulates in the aqueous phase.