TABLE 1.

Detected lipid (sub)classes and lipid species of the TL, IM, and OM fraction of the C. glutamicum WT, TmaT mutant, and PBQC via LC-MS/MS ESI TOF in positive ionization mode (CE30)

			Number of Lipid Species
			WT			TmaT KO Mutant			PBQC
	Lipid (Sub)Class	RT (min)	TL	IM	OM	TL	IM	OM	TL	IM	OM	Total Number
1	hTMCM	4.6–12.6	19	18	20	26	26	15	26	22	19	33
2	AcTMCM	6.6–11.5	4	3	8	0	0	0	6	5	7	8
3	keto-TMCM	6.0–9.0	4	3	0	10	10	3	9	7	2	11
4	Acyl-hTMCM	15–19	10	7	5	12	12	5	11	11	6	14
5	Acyl-AcTMCM	16.9–19.3	1	1	0	0	0	0	1	1	0	1
6	h2TDCM	20.0–22.0	30	28	28	22	30	25	29	29	23	43
7	Ac1-hTDCM	21.3–21.6	3	3	3	2	3	1	3	3	3	3
8	hGMM	6.5–13.0	9	5	11	6	8	7	8	7	7	12
9	DAG	11–14.5	7	7	4	8	8	4	7	8	4	8
10	Ala-DAG	10–13.5	13	11	5	9	9	2	12	12	4	13
11	CDP-DAG	6–7	1	1	0	0	1	0	0	1	0	1
12	TAG	20.0–22.0	9	10	10	10	11	11	10	9	10	13
13	PG	5.9–10.1	11	11	5	5	5	2	10	10	5	11
14	Acyl-PG	14–15	3	3	0	1	1	0	2	2	0	3
15	Ala-PG	9–10	2	2	1	1	1	0	2	2	1	2
16	PA	8.6–9.2	2	2	0	1	0	0	1	0	0	2
17	CL	17–21	16	16	3	14	14	4	14	14	3	18
18	PI	6.7–9.8	4	4	3	2	3	2	4	4	2	6
19	PIM1	5.9–10.2	1	1	0	1	1	0	1	1	0	1
20	PIM2	7.0	1	1	1	1	1	1	1	1	1	1
21	AcPIM2		5	4	1	6	5	1	4	5	1	7
22	AcPIM3	12–14.5	1	1	0	1	1	0	1	1	0	1
23	AcPIM4		0	1	0	1	1	0	0	1	0	1
24	Gl-A	5.6–10	7	6	2	6	5	1	7	5	1	8
25	Gl-X	5.7–8.4	6	6	1	4	4	1	4	5	1	6
26	Gl-Y	8.1	0	1	0	1	1	1	1	1	0	1
27	Gl-Z	7.9	1	1	0	1	1	0	1	1	0	1
28	Acyl-PG-like	14.0–17.0	4	4	3	0	0	0	3	3	3	4
	Sum:		174	161	114	151	162	86	178	171	103	233