TABLE 1.

Characterization of rHDL

rHDL	POPCa	FCa	APOA1b	Particle Diameter (nm)c	Particle Diameter (nm) EMd	Helicity (%)e
WT	102.1 ± 3.9	7.7 ± 3.8	1.0	9.6	10.3 ± 1.6	82.5 ± 2.2
K133C	92.6 ± 5.2	6.9 ± 2.6	1.0	9.6	10.5 ± 1.2	82.6 ± 2.0
K206C	94.7 ± 8.4	6.9 ± 1.8	1.0	9.5	10.2 ± 1.4	81.6 ± 3.0
K195C	94.4 ± 4.9	7.5 ± 1.7	1.0	9.6	9.1 ± 1.4	83.4 ± 4.1

rHDL particles were reconstituted at an initial molar ratio of 80:8:1 POPC:FC:APOA1. Results shown indicate mean ± SD of at least three independent rHDL preparations. Calculations represent scans of three independent samples of rHDL preparations. FC, free cholesterol.

^aPhospholipid and cholesterol concentrations determined as indicated in the Materials and Methods.

^bAPOA1 concentration was determined using a Markwell Protein Lowry.

^cParticle size determined using NDGGE by comparison to protein standards.

^dParticle size determined by EM.

^ePercent α-helicity determined by CD.