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LPLA2 variant (WT, D13F, D13A) transacylase activities with SLPC, SOPC and OSPC at pH 4.5. Liposomes containing of SLPC, SOPC, or OSPC as substrates with sulfatide and NAS were incubated with purified LPLA2 variants for 5, 15, and 30 min at 37°C. The transacylase activities of different LPLA2 variants at 30 min using SLPC (A and B), SOPC (C and D), and OSPC (E and F) as substrate. The histogram values represent the mean ± SD (n = 3), *P < 0.05, **P < 0.01, ***P < 0.001.
