Fig. 5.

LPLA₂-D13F transacylase activity with SLPC, SOPC, and OSPC substrates at pH 4.5 and 7.4. Liposomes containing SLPC, SOPC, OSPC, and SLPC/SOPC (1:1ratio) along with sulfatide, and NAS (10:1:3 molar ratio) were incubatedwith 30 ng protein of the D13F variant for 5, 15, and 30 min at 37°C at pH 4.5 and 7.4. The reaction 1-O-Acyl-NAS products were separated by HPTLC (A and B, respectively). The expression of sn-2/sn-1 transacylase activity of D13F at 30 min at pH 4.5 and pH 7.4 using SLPC, SOPC, OSPC (C), and mixed liposomes SLPC/SOPC (D) for acyl donor. The graph values represent the mean ± SD (n = 3) per time point using a t-test, *P < 0.05, **P < 0.01, ***P < 0.001.