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. 2018 May 3;59(7):1205–1218. doi: 10.1194/jlr.M084012

Fig. 6.

Fig. 6.

LPLA2 variant D13F transacylase activity with PLPC, POPC, and OPPC at pH 4.5 and 7.4. Liposomes containing of SLPC PLPC, POPC, and OPPC/sulfatide/ NAS (10:1:3 molar ratio) were incubated with purified D13F variant (30 ng, pH 4.5; 60 ng, pH 7.4) for 5, 15, and 30 min at 37°C. The reaction products were separated by argentation HPTLC using a solvent system consisting of chloroform/acetic acid/methanol (90:5:1). All histograms represent the mean of the LPLA2 transacylase activity ± SD (n = 3). A, B: 1-O-Acyl production of D13F using liposomes consisting of POPC and OPPC at pH 4.5 and 7.4. C, D: 1-O-Acyl production of D13F at pH 4.5 using liposomes consisting of SLPC or PLPC/sulfatide/NAS (10:1:3 molar ratio). E, F: Same conditions as in C and D except the transacylation reaction was conducted at pH 7.4. The graph values represent the mean ± SD (n = 3) per time point using a t-test, *P < 0.05, **P < 0.01, ***P < 0.001.