TABLE 1.

Thermal stability and membrane binding of LPLA₂ variants at pH 4.5 and 7.4

LPLA2 variant	Tm°C	ΔTm	Binding at pH 4.5 (% WT)	Binding at pH 7.4 (%WT at pH 4.5)
WT	65.6 ± 1.4	—	100 ± 6.5	9.5 ± 1.3
D13A	64.5 ± 1.0	−1.1	86 ± 8.7*	16.0 ± 3.0*
D13C	64.9 ± 2.5	−0.7	69 ± 8.3*	9.0 ± 1.8
D13F	63.3 ± 1.0	−2.3	93 ± 11	20.0 ± 1.6**
To LCC	61.3 ± 2.0	−4.3	69 ± 5.0*	9.0 ± 2.6
To LCF	57.3 ± 1.3	−8.3	68 ± 22*	10.0 ± 3.4

T_m is defined as the inflection point of the melting curve and was calculated by the Boltzmann equation (32). Membrane binding was measured by the liposome pull-down assay as described in the Methods. The data represent the mean ± SD (n = 3) per measurement, *P < 0.05, **P < 0.001 using a t-test.