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. 2018 May 8;59(7):1164–1174. doi: 10.1194/jlr.M081398

Fig. 4.

Fig. 4.

Gonadectomy increases levels of hepatic Cer(d18:1/16:0) and Cer(d18:1/18:0) in male mice. Hepatic Cer(d18:1/16:0) (A), Cer(d18:1/18:0) (C), and Cer(d18:1/20:0) (E) levels in C57BL/6J, C3H/HeJ, and DBA/2J male mice after gonadectomy (GDX), with or without testosterone implants or sham-operated controls. Hepatic Cer(d18:1/16:0) (B), Cer(d18:1/18:0) (D), and Cer(d18:1/20:0) (F) levels in C57BL/6J, C3H/HeJ, and DBA/2J female mice after ovariectomy (GDX) or sham-operated controls. Hepatic mRNA expression of Cers6 in male (G), and female (H) mice. The mice were maintained on a chow diet from weaning until 8 weeks of age before switching to a HF/HS diet for another 8 weeks. Results are presented as mean ± SEM. Statistical differences between groups were tested using unpaired two-tailed Student’s t-tests (n = 3–4 mice per strain, group, and sex). *P < 0.05 and **P < 0.01 between the gonadectomized group and sham-operated controls. $P < 0.05 and $$P < 0.01 between gonadectomized males and gonadectomized males with testosterone implants. The ceramides were measured with HPLC coupled to TOF-MS.