TABLE 1.
Gonadectomy increases hepatic mRNA levels of several genes encoding enzymes in ceramide metabolism
| Males | Females | |||
| Sham | GDX | Sham | GDX | |
| Sptlc1 | 12.7 (0.6) | 15.4 (1.0)a | 15.7 (0.8)b | 14.4 (0.2) |
| Sptlc2 | 15.8 (1.1) | 16.8 (0.2) | 18.1 (1.5) | 19.5 (1.3) |
| Cers2 | 230.9 (6.1) | 199.5 (7.5)c | 193.6 (3.6)d | 194.9 (8.6) |
| Cers4 | 2.4 (0.3) | 2.5 (0.4) | 1.7 (0.3) | 2.4 (0.3) |
| Cers5 | 1.2 (0.1) | 2.1 (0.4) | 2.3 (0.3)b | 2.3 (0.3) |
| Cers6 | 10.7 (0.7) | 23.5 (1.4)e | 23.8 (1.5)f | 23.8 (1.4) |
| Degs1 | 48.2 (1.9) | 55.2 (1.5)a | 55.8 (1.1)b | 52.1 (3.1) |
| Asah1 | 23.4 (1.9) | 28.5 (0.9)a | 30.7 (1.8)b | 32.4 (1.8) |
| Ugcg | 13.8 (0.3) | 18.1 (2.3) | 21.2 (0.6)f | 22.1 (1.8) |
| Cerk | 1.9 (0.1) | 2.7 (0.2)c | 2.7 (0.3) | 3.0 (0.5) |
| Kdsr | 7.9 (0.2) | 12.0 (0.6)c | 12.3 (0.7)d | 12.8 (0.6) |
| Smpd1 | 25.2 (0.4) | 26.1 (0.8) | 22.3 (0.1)d | 25.3 (2.9) |
mRNA values are expressed as fragments per kilobase of transcript per million mapped reads (FPKM) (32). Results are presented as mean ± SEM.
a
P < 0.05 between the gonadectomized group and sham-operated controls.
b
P < 0.05 between the sexes.
c
P < 0.01 between the gonadectomized group and sham-operated controls.
d
P < 0.01 between the sexes.
e
P < 0.001 between the gonadectomized group and sham-operated controls.
f
P < 0.001 between the sexes.