Figure 2.

ISG15 mediates IFN-$\alpha$ sensitivity in HCC cells. Western blotting was used to detect PARP cleavage and ISG15 protein expression in (A) LH86 and (B) Huh7 cells after treatment with 2,000 IU/mL IFN-$\alpha$ for 48 h. Apoptosis in (C) LH86 and (D) Huh7 cells was assessed using flow cytometry after treatment with 2,000 IU/mL IFN-$\alpha$ for 48 h. The data presented are representative of 3 independent experiments.