Figure 3.

Identification of miR-370 target sequence in ISG15 mRNA, and analysis of miR-370 expression in HCC tumor tissues and cell lines. (A) The 3${}^{\prime }$-UTR of ISG15 mRNA contains one predicted miR-370 binding site, as indicated in the alignment of the seed region of miR-370 with ISG15 3${}^{\prime }$-UTR sequence. Relative quantification of the level of miR-370 in (B) HCC tumor samples (**$P<$ 0.01 compared to adjacent noncancerous tissues) and (C) HCC cell lines (**$P<$ 0.05 and *$P<$ 0.05 compared with LO2 and LH86 cells, respectively) was performed using qRT-PCR, and the results were normalized based on U6 expression. The data presented are representative of 3 independent experiments.