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. 2018 Jun 19;22(3):453–466. doi: 10.3233/CBM-171075

Figure 4.

Figure 4.

Effects of miR-370 on ISG15 expression and PARP cleavage. (A) Huh7 and LH86 cells were transfected with 25, 50 or 100 nmol miR-370 or anti-miR-370 siRNA for 24 h before assessing the levels of ISG15 and actin (loading control) proteins by western blotting. (B) After transfection with 100 nmol miR-370 and/or pFLAG-ISG15 for 24 h, LH86 and Huh7 cells were subjected to western blotting to assess the levels of cleaved PARP, ISG15, and actin proteins. (C and D) After treatment with or without 2,000 IU/mL IFN-α for 48 h, Huh7 and LH86 cells were transfected with 100 nmol miR-370 or anti-miR-370 siRNA for 24 h, and the levels of cleaved PARP, ISG15, and actin proteins were assessed by western blotting. The data presented are representative of 3 independent experiments.