Table S1.
The bioinformatic results of miR-370 downregulates ISG15 expression by targeting the 3’-UTR of the ISG15
| Predicted consequential pairing of | Seed | Site-type | 3’pairing | Local AU | Position | TA | SPS | Context | Context score | Conserved | Per | |
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| target region (top) and miRNA (bottom) | match | contribution | contribution | contribution | contribution | contribution | contribution | score | percentile | branch length | ||
| Position 3–9 of ISG15 has-miR-650 | 5’ NNNNNNNNNNNNNNG 3’ CAGGACUCUCGCG | 7mer-1A | 0.074 | NA | NA | NA | NA | NA | NA | Too close to ORF | 0.073 | NA |
| Position 3–9 of ISG15 has-miR-4443 | 5’ NNNNNNNNNNNNNNG 3’ UUUUGGGU | 7mer-m8 | 0.120 | NA | NA | NA | NA | NA | NA | Too close to ORF | 0.073 | NA |
| Position 3–9 of ISG15 has-miR-3612 | 5’NNNNNNNNNNNNNNG 3’AGGUAAAGAGUUCU | 7mer-1A | 0.074 | NA | NA | NA | NA | NA | NA | Too close to ORF | 0.073 | NA |
| Position 6–12 of ISG15 has-miR-3615 | 5’NNNNNNNNNNNGGGC 3’ACUCCAGUGUAACG | 7mer-1A | 0.074 | NA | NA | NA | NA | NA | NA | Too close to ORF | 0.073 | NA |
| Position 7–13 of ISG15 has-miR-4456 | 5’NNNNNNNNNNGGGCC 3’UUUUCCUU | 7mer-m8 | 0.120 | NA | NA | NA | NA | NA | NA | Too close to ORF | 0.343 | NA |
| Position 8–15 of ISG15 has-miR-138 | 5’ NNNNNNNNNGG 3’GCCGGACUAA | 8mer | 0.247 | NA | NA | NA | NA | NA | NA | Too close to ORF | 0.343 | 0.1 |
| Position 17–23 of ISG15 has-miR-423-3p | 5’GGCCUCCA 3’UGACUCCCCGGAGUC | 7mer-1A | 0.074 | 0.006 | 0.034 | 0.048 | 0.08 | 0.085 | 0.26 | 71 | 0.013 | NA |
| Position 20–26 of ISG15 has-miR-370 | 5’CUCCACCAG 3’UGGUCCAAG | 7mer-1A | 0.074 | 0.002 | 0.042 | 0.048 | 0.02 | 0.063 | 0.13 | 59 | 0.013 | NA |
| Position 24–30 of ISG15 has-miR-640 | 5’ACCAGCAUCCGAGCA 3’UCUCCGUCCAAGGA | 7mer-1A | 0.074 | 0.002 | 0.053 | 0.048 | 0.015 | 0.022 | 0.11 | 52 | 0.368 | NA |
| Position 27–33 of ISG15 has-miR-4780 | 5’AGCAUCCGAGCAGGA 3’CGAUCCCUAGUCCG | 7mer-m8 | 0.120 | 0.007 | 0.048 | 0.058 | 0.01 | 0.031 | 0.18 | 76 | 0.073 | NA |
| Position 33–40 of ISG15 has-miR-1180 | 5’CGAGCAGGAUCAA 3’UGUGUGGGUGCG | 8mer | 0.247 | 0.003 | 0.043 | 0.109 | 0.137 | 0.114 | 0.56 | 97 | 0.013 | NA |
| Position 34–40 of ISG15 has-miR-4664-3p | 5’GAGCAGGAUCAAGGG 3’CUGCCCCGAGUGUCU | 7mer-1A | 0.074 | 0.002 | 0.013 | 0.048 | 0.088 | 0.044 | 0.24 | 67 | 0.013 | NA |
| Position 54–61 of ISG15 has-miR-4753-3p | 5’AAUAAAGGCUGUUGU 3’UGUGUUCCGAUUUC | 8mer | 0.247 | 0.060 | 0.008 | 0.114 | 0.054 | 0.078 | 0.30 | 99 | 0.406 | NA |