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. Author manuscript; available in PMC: 2018 Dec 25.
Published in final edited form as: Nat Microbiol. 2018 Jun 25;3(7):814–823. doi: 10.1038/s41564-018-0177-8

Figure 3. In vitro culture of C. parvum in human lung organoids.

Figure 3

(a) (left) Schematic representation showing microinjection. (right) C. parvum 18S rRNA was measured at each time point after injection in lung organoids by qRT-PCR (n=2 biologically independent samples). Mean value at each time point was used for connecting line.

(b) Immunofluorescence of meront I and microgamont stages in lung organoids. Anti-gp-15 antibody (yellow) marks both merozoites in meront and microgametes in microgamont. Scale bars indicate 5μm. More than 20 meronts I and 5 microgamonts were observed.

(c) Immunofluorescence of newly formed oocysts inside lung organoids at 168 hr post-injection. Scale bars indicate 20μm. The oocysts were observed from more than 6 independent organoids. Media-injected organoids were used as a control.