Table 2. Putative novel miRNAs with significant changes after ethanol exposure.
| miRNA | Δ EtOH | Fold-change | P-value | Sequence | Chromosomal location |
|---|---|---|---|---|---|
| novel_miR_1 | Up | 10.92 | 2.93E-102 | GCGUCGGUGGUGUAAUGGUUAG | 3R 2955586:2955607 |
| novel_miR_2 | Up | 8.09 | 5.14E-15 | GUGGGCGGUGGAGAGGUGGAG | 3L 935306:935326 |
| novel_miR_3 | Up | 8.09 | 5.14E-15 | GUGGGUGGGGUGGACGGGCA | X 9176644:9176663 |
| novel_miR_4 | Up | 7.51 | 2.98E-10 | UCGGUCCACCCGGGGGCGCCA | 2L 1856619:1856599 / 2R 9632238:9632218 |
| novel_miR_5 | Up | 7.09 | 7.19E-08 | UUGGGGGGCGCGGGUGGUGG | X 9057913:9057893 |
| novel_miR_6 | Down | -1.02 | 6.81E-03 | AGAGCGACGAAGACUGAGCUG | 3L 14420650:14420630 |
| novel_miR_7 | Down | -6.78 | 1.64E-06 | CCGGGUGUGUGUGGAUGGGUA | 3R 21410603:21410623 |
| novel_miR_8 | Down | -7.11 | 4.93E-08 | UUGGGGGGCGCGGGUGGUGGC | X 9057913:9057893 |
| novel_miR_9 | Down | -7.17 | 2.45E-08 | UGUGGGCAGGCGAAUGAUGUGG | 2L 4458246:4458225 |
| novel_miR_10 | Down | -7.34 | 2.99E-09 | GUUGAACGGUCGUUGAACAAU | X 13635866:13635886 |
| novel_miR_11 | Down | -7.43 | 7.36E-10 | GCGGGUCGGAGUGGGUGGUGG | 2L 16283135:16283115 |
| novel_miR_12 | Down | -9.05 | 9.98E-29 | UUGGGCGGGGACGGGCGGCGG | 3R 3300451:3300431 |
| novel_miR_13 | Down | -10.82 | 5.54E-96 | GUGGGAGCGGUGGGCGGAGCG | 3L 4576278:4576298 |
Ethanol-induced changes are shown relative to control. Fold change is expressed as log2 values (EtOH/Ctrl). The relative abundance of each miRNA, measured as normalized reads per million before and after ethanol treatment, can be found in the Supplementary Data 1. All of these match criteria for a miRNA (see Methods) but none have been functionally tested to confirm that functionally act as a miRNA.