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. 2018 May 28;37(13):e98354. doi: 10.15252/embj.201798354

Figure EV3. Partial inhibition of protein synthesis by redaporfin‐PDT .

Figure EV3

  • A, B
    Representative images of newly synthesized proteins evaluated by detection of L‐azidohomoalanine in U2OS cells submitted to PDT with redaporfin (5 μM) in methionine‐free conditions and cycloheximide (CHX, 50 μM) (A), and quantitative analysis that depicts fluorescence signal on the cytoplasm (B).
Data information: Ctr indicates untreated cells and Redp* indicates irradiated cells. Data are indicated as means ± SD of triplicates of one representative experiment out of 2–4 repeats. Asterisks indicate significant differences with respect to untreated cells, ***P < 0.001 (one‐way ANOVA). Scale bars: 10 μm.